Impact of Human Immunodeficiency Virus Type 1 (HIV-1) Genetic Diversity on Performance of Four Commercial Viral Load Assays: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR v1.5, VERSANT HIV-1 RNA 3.0, and NucliSens HIV-1 QT
نویسندگان
چکیده
منابع مشابه
Comparison of the artus HIV-1 QS-RGQ and VERSANT HIV-1 RNA 1.0 assays for quantitative detection of human immunodeficiency virus type 1 in plasma samples.
Several integrated diagnostic platforms to quantify human immunodeficiency virus type-1 viremia have been developed in recent years. We evaluated the performances of the Artus HIV-1 QS-RGQ assay, using the complete QIAsymphony RGQ workflow. 192 clinical plasma specimens and external control panel samples were analyzed, using the Artus assay and the routine Siemens VERSANT HIV-1 RNA 1.0 assay. T...
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چکید ه سابقه و هدف با شروع عفونت HIV-1 ، اولین مارکری که در خون آشکار می‌شود، RNA ویروسی می‌باشد. از میان روش‌های موجود که شناسایی RNA را هدف قرار می‌دهند؛ متداول‌ترین روش‌ها NASBA و RT-PCR هستند. NASBA مزیت‌های متعددی نسبت به RT-PCR ارایه می‌کند. در این پژوهش، واکنش NASBA در ترکیب با روش آشکارسازی آسان و حساس الایزا، برای تشخیص و آشکارسازی HIV-1 RNA مورد استفاده قرار گرفت. مواد و روش‌ه...
متن کاملQuantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay.
HIV-1 RNA viral load has become the major biological marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The aim of this study was to compare the performance of the new CE marked NucliSens EasyQ HIV-1 assay with NucliSens HIV-1 QT assay (reference method). NucliSens EasyQ HIV-1 (EasyQ) couples nucleic acid sequence-based amplification...
متن کاملVenom Components of Iranian Scorpion Hemiscorpius lepturus Inhibit the Growth and Replication of Human Immunodeficiency Virus 1 (HIV-1)
Background: During the recent years, significant progress has been achieved on development of novel anti-viral drugs. Natural products are assumed as the potential sources of novel anti-viral drugs; therefore, there are some previous studies reporting the anti-viral compounds from venomous animals. Based on the significant value for tracing of non-toxic anti-viral agents from natural resources,...
متن کاملEffect of HIV-1 genetic diversity on HIV-1 RNA quantification in plasma: comparative evaluation of three commercial assays.
To the Editor: The rapid rate of HIV-I replication in vivo (10' virions per day), coupled with the poor fidelity of reverse transcription of the HIV-1 genome, results in the production of new virus variants (1). This makes it easy to understand how multiple clades of HIV-1 have emerged throughout the world and explains the development of genetic diversity even within clades (2). Numerous report...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2005
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.43.8.3860-3868.2005